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In the present study the genomic identification and characterization of the Sus2-2A and Sus2-2B genes coding for SUS in durum wheat (cultivars Ciccio and Svevo) is reported.

The activity of the encoded proteins was evaluated by specific activity assays on endosperm extracts and their structure established by modeling approaches. doi: 10.1016/20 Pub Med Abstract | Cross Ref Full Text | Google Scholar Zheng, Y., Anderson, S., Zhang, Y., and Garavito, R. A complete set of nulli-tetrasomic lines (NTs) developed by Sears (1966) from the common wheat (Triticum aestivum L. aestivum) cv Chinese Spring were used to physically localize Sus2 markers to specific chromosomes. In order to identify the complete genomic sequences of the three Ta Sus2 genes in hexaploid wheat, an available Chinese Spring genomic sequence in the cereals database was used. Integrated genomic and proteomic analyses of gene expression in mammalian cells. The first one is the structural gene for the major endosperm isoform of the enzyme that is anaerobically induced in roots and shoots, while the second is the structural gene for the constitutively expressed isoenzyme in embryo and other tissues.Similar genes corresponding to the SUS1 and SUS2 proteins are also present in barley (Sánchez de la Hoz et al., 1992) and in wheat (Martinez de Ilarduya et al., 1993).

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